Human IgA Mouse Monoclonal Antibody [Clone ID: NI 69 (A89-034) and NI 184 (A89-035)]

Specifications

Product Data
Clone Name	NI 69 (A89-034) and NI 184 (A89-035)
Applications	ELISA, IF, IP, WB
Recommended Dilution	To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used. General Recommended Dilutions: Histochemical Use: 1/10-1/50. ELISA: from 1/50 upwards. Western blot: from 1/100 upwards.
Reactivities	Human
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Highly purified monoclonal IgA isolated from pooled human serum
Specificity	The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA. It reacts with both subclasses of IgA as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Formulation	PBS, pH 7.2 without preservatives or foreign proteins. Label: HRP State: Lyophilized purified Ig fraction Label: Horseradish Peroxidase Molar radio: Peroxidase/IgG ~1.7
Reconstitution Method	Restore by adding 0.5 ml sterile distilled water.
Concentration	0.4 mg/ml
Conjugation	HRP
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.