Human IgA (alpha chain) rabbit polyclonal antibody, TRITC
Specifications
Product Data
Recommended Dilution
This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF Microscopy: 1/1,000-1/5,000. Flow Cytometry: 1/500-1/2,500.
Reactivity
Human
Antibody Host
Rabbit
Clonality
Polyclonal
Immunogen
Human IgA alpha chain.
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgA and Human Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Buffer
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: TRITC State: Lyophilized purified Ig fraction. Label: Tetramethylrhodamine isothiocyanante ; Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 2.3 moles TRITC per mole of Rabbit IgG.
Reconstitution Method
Restore with 1.0 ml of deionized water (or equivalent).
Concentration
lot specific
Purification
Immunoaffinity Chromatography.
Conjugation
TRITC
Storage
Store vial at 2-8°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
