HLA Class I ABC Mouse Monoclonal Antibody [Clone ID: W6/32]
Katalog-Nummer SM1222LE
Size : 500ug
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HLA Class I ABC Mouse Monoclonal Antibody [Clone ID: W6/32]
| Product Data | |
| Clone Name | W6/32 |
|---|---|
| Application | ELISA, FC, FN, IF, IHC, IP |
| Recommended Dilution | ELISA. Immunoprecipitation (Ref 1). Functional Assays. Immunofluoresecnce. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl. This product is routinely tested in Flow Cytometry on Human peripheral blood lymphocytes. Immunohistochemistry on Frozen Sections (See Ref 6). Positive Control Tissue: Tonsil. Not suitable for Immunohistochemistry on Paraffin Sections. |
| Reactivity | Baboon, Bovine, Chimpanzee, Feline, Human, Macaque, Monkey, Primate |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | Purified Human tonsil lymphocyte membranes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line |
| Specificity | SM1222LE recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha-3 and alpha-2 domains (Ref 7). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. |
| Buffer | PBS without preservatives or stabilizers. Endotoxin level: Less than 0.01 EU/µg State: Low Endotoxin State: Liquid purified IgG fraction |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at -20°C only. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch |
| Synonyms | MHC class I ABC, HLA Class 2 ABC |
| Reference Data | |