Histone H2B Mouse Monoclonal Antibody [Clone ID: C14264]
Specifications
Product Data | |
Clone Name | C14264 |
Applications | IF, IP, WB |
Recommended Dilution | Western blot: 1 μg/ml for chemiluminescence detection system. Immunoprecipitation: 2 μg/200 μl of cell extract from 5x106 cells. Immunocytochemistry: 1 μg/ml. For details see Protocols below. |
Reactivities | Hamster, Human, Mouse, Rat |
Host | Mouse |
Isotype | IgG2b |
Clonality | Monoclonal |
Immunogen | Protein fraction extracted from the mitotic chromosome |
Specificity | This antibody reacts with Histone H2B. |
Formulation | PBS containing 50% Glycerol, pH 7.2, without preservatives State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A Agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Predicted Protein Size | 14 kDa |
Background | The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes multiple different post-translational modifications including acetylation, phosphorylation, ubiquitination, methylation, and ADP-ribosylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. Acetylation of histone H2B was suppressed to greater extent than that of the others, with histone H3 being relatively least affected. The analysis of acetylation status of each of the four lysine sites at the N-terminal tail of histone H2B revealed decreases consistent with those observed in the total acetylation patterns, with the K12 and K20 residues being markedly more affected than K5 and K15 residues. |
Synonyms | H2B Histone Family, HIST1H2B |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting Immunoprecipitation Immunofluorescence microscopy |
Reference Data |
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