Application
| WB, IHC, AM |
---|---|
Primary Accession | Q9JKA7 |
Other Accession | NP_067690.1 |
Host | Mouse |
Isotype | IgG1 |
Reactivity | Human, Mouse, Rat, Rabbit |
Clonality | Monoclonal |
Description | Mouse Anti-Rat HCN4 Monoclonal IgG1 |
Target/Specificity | Detects ~130kDa. No cross-reactivity against other HCNs. |
Other Names | Hyperpolarization activated cyclic nucleotide gated potassium channel 4 Antibody, HCN 4 Antibody, Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4 Antibody |
Clone Names | S114-10 |
Immunogen | Fusion protein amino acids 1-019-1198 (c-terminus) of rat HCN4 |
Purification | Protein G Purified |
Storage | -20ºC |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | 1 µg/ml of SMC-320 was sufficient for detection of HCN4 in 10 µg of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Cellular Localization | Membrane |
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (1).
References
1. Zong X., et al. (2005) J Biol Chem. 280(40): 34224-34232
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Cat# ASM10198