Gosr2 (NM_019650) Mouse Tagged ORF Clone

Katalog-Nummer MR202303

Size : 10ug

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Gosr2 (NM_019650) Mouse Tagged ORF Clone

SKU
MR202303
Gosr2 (Myc-DDK-tagged) - Mouse golgi SNAP receptor complex member 2 (Gosr2)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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Specifications
Product Data
Target Symbol Gosr2
Synonyms 2310032N09Rik; C76855; Gs27; membrin; SNARE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202303 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCCTATACCAACAAACGAACAAGCAGGTTCAGGAGATCCAGTCTCACATGGGACGCCTGGAGA
GGGCAGACAAGCAGTCTGTGCACTTAGTAGAAAATGAGATCCAAGCAAGCATAGAGCAAATATTCAGCCA
TCTGGAGCGCCTGGAGATTTTGTCAAGCAAGGAGCCCTTGAATAGAAGACAGAATGCCAAACTCCGTGTC
GACCAGCTAAAGTATGATGTCCAGCACTTGCAGACTGCCCTCAGGAACTTTCAGCACCGGCGCCAAGTCA
GGGAGCAGCAGGAGAGACAGCGAGACGAGCTTCTGTCTCGCACCTTCACCACTAACGACTCTGACACCAC
CATTCCCATGGATGAATCACTGCAGTTTAACTCCTCCCTCCACAACATTCACCACGGCATGGATGACCTC
ATTGGAGGCGGGCACAGTATTCTGGAGGGACTGAGGGCCCAGAGACTGACCTTGAAGGGGACTCAGAAGA
AGATCCTTGACATTGCTAACATGCTCGGCTTGTCCAACACAGTGATGAGGCTGATTGAGAAGCGGGCCTT
CCAGGACAAGTACTTCATGATCGGTGGGATGCTGCTCACTTGTGCAGTCATGTTCCTCGTGGTACAGTAC
CTGACA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR202303 protein sequence
Red=Cloning site Green=Tags(s)

MEPLYQQTNKQVQEIQSHMGRLERADKQSVHLVENEIQASIEQIFSHLERLEILSSKEPLNRRQNAKLRV
DQLKYDVQHLQTALRNFQHRRQVREQQERQRDELLSRTFTTNDSDTTIPMDESLQFNSSLHNIHHGMDDL
IGGGHSILEGLRAQRLTLKGTQKKILDIANMLGLSNTVMRLIEKRAFQDKYFMIGGMLLTCAVMFLVVQY
LT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_019650
ORF Size 639 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_019650.4
RefSeq Size 3078 bp
RefSeq ORF 639 bp
Locus ID 56494
UniProt ID O35166
Cytogenetics 11 67.43 cM
MW 24.7 kDa
Summary Involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.[UniProtKB/Swiss-Prot Function]
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SKU Description Size
MC200583 Gosr2 (untagged) - Mouse golgi SNAP receptor complex member 2 (Gosr2), (10ug) 10 ug
MG202303 Gosr2 (tGFP-tagged) - Mouse golgi SNAP receptor complex member 2 (Gosr2) 10 ug
MR202303L3 Lenti ORF clone of Gosr2 (Myc-DDK-tagged) - Mouse golgi SNAP receptor complex member 2 (Gosr2) 10 ug
MR202303L4 Lenti ORF clone of Gosr2 (mGFP-tagged) - Mouse golgi SNAP receptor complex member 2 (Gosr2) 10 ug
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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Katalog-Nummer
Beschreibung
Cond.
Preis zzgl. MwSt.
A2585-10mg
 10mg