Goat anti-Human IgG (H&L), ALP conjugated
Katalog-Nummer AS10762
Size : 1mg
Marke : Agrisera
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Goat anti-Human IgG (H&L), ALP conjugated
Product no: AS10 762
AS10 762 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG (heavy and light chains - H&L)
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| Immunogen: | Purified human IgG |
| Host: | Goat |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified goat IgG. |
| Format: | Liquid |
| Quantity: | 1 mg |
| Storage: | Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
| Tested applications: | ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB) |
| Recommended dilution: | 1 : 500-1 :2000 (ELISA) , 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB) |
| Confirmed reactivity: | Human IgG (H&L) |
| Predicted reactivity: | Human IgG (H&L) |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
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| Additional information: | ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative |
| Additional information (application): | This antibody reacts with alpha (heavy chains) on human IgG and all light chains on human immunoglobulins based on immunoelectrophoresis. No reactivity is observed to non-immunoglobulin human serum proteins based in immunoelectrophoresis. |
| Background: | Goat anti-human IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays. |