Goat anti-Guinea pig IgG (H&L), HRP conjugated
Katalog-Nummer AS10653
Size : 1mg
Marke : Agrisera
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Goat anti-Guinea pig IgG (H&L), HRP conjugated
Product no: AS10 653
AS10 653 | Clonality: Polyclonal | Host: Goat | Reactivity: Guinea pig IgG (H&L)
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| Immunogen: | Purified Guinea pig IgG, whole molecule |
| Host: | Goat |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified goat IgG. |
| Format: | Lyophilized |
| Quantity: | 1 mg |
| Reconstitution: | For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily |
| Storage: | Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
| Tested applications: | ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB) |
| Recommended dilution: | 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB) |
| Confirmed reactivity: | Heavy chains on guinea pig IgG and light chains on all guinea pig immunoglobulins |
| Not reactive in: | Non-immunoglobulin guinea pig serum proteins |
| Additional information: | HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 10 % (w/v) BSA, Protease/IgG free with 0,1 % (v/v) of Kathon CG as preservative |
| Background: | Goat anti-guinea pig IgG is a secondary antibody conjugated to HRP which binds to all guinea pig IgGs in immunological assays. |
| Selected references: | Jin et al. (2019). YAP activation promotes the transdifferentiation of cardiac fibroblasts to myofibroblasts in matrix remodeling of dilated cardiomyopathy. Brazilian Journal of Medical and Biological Research (2019) 52(1): e7914. |
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