Glutathione S-Transferase Yb2, Rat (GST)

Each vial contains sufficient enzyme for 2 assays using CDNB as substrate in a spectrophotometric assay.||Source:|Rat Liver||Specific Activity:|5.9units/mg||Applications:|Suitable for use as a standard for Western blotting or for ELISAs. Other applications not tested.|Optimum dilutions to be determined by researcher.||Definition of unit:|One unit is the amount of enzyme that catalyzes the conjugation of 1uM of 1-chloro-2,4-dinitrobenzene (CDNB) to glutathione in one minute at room temperature in 100mM potassium phosphate buffer, pH 6.5. [J.B.C., 249 : 7130-7139 (1974)]||Storage and Stability:|Stable indefinitely at -70°C. Labile to repeated freezing and thawing.