F(ab')2 Anti-Rat IgM (mu chain) (Phycoerythrin Conjugated) Secondary Antibody

Katalog-Nummer ASR3199

Size : 1mL

Marke : Abcepta

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Product Information
Description F(ab')2 Anti-RAT IgM (mu chain) (GOAT) Antibody Phycoerythrin conjugated
Host Goat
Conjugate R-Phycoerythrin (RPE)
Target Species Rat
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, FC
Application Note IF Microscopy 1:100-1:250
FlowCytometry 1:100-1:250
FLOW1:100-1:250
Physical State Lyophilized
Host Isotype IgG F(ab')2
Target Isotype IgM µ chain
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rat IgM mu heavy chain
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgM, and Rat Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or human, bovine or horse serum proteins.
Storage Condition Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.