This product is suitable for isolating miRNA and total RNA from cells, tissues, fresh blood and exosomes. After samples are lysed  with lysis buffer, addition of chloroform to the lysed sample separates the solution to an upper colorless aqueous phase containing  RNA, an interphase and a lower pink organic phase. After addition of ethanol to the transferred aqueous phase, total RNA can be  specifically immobilized by an RNA Spin Column. By adjusting the volume of ethanol added to the aqueous phase, large RNAs  (including 28S rRNA, 18S rRNA and mRNA) are immobilized while small RNAs (≤ 200 nt, such as miRNA, siRNA, shRNA,  snRNA, etc.) are in the flow-through which is passed through a miRNA Spin Column where the miRNAs become immobilized after  addition of more ethanol. This product possesses advantages of high lysis capacity, high yield and broad applications.

Storage

LB10 at 2-8℃ away from light for one year; others at room temperature (15℃-25℃) in a dry place for one year.

Shipping
LB10 on ice bag (4 °C); others at room temperature.

Wu H , Li Z , Yang P , et al. PKM2 depletion induces the compensation of glutaminolysis through β-catenin/c-Myc pathway in tumor cells[J]. Cellular Signalling, 2014, 26(11):2397-2405.

Zhang J , Xu Y , Cao T , et al. Arsenic methylation by a genetically engineered Rhizobium-legume symbiont[J]. Plant and Soil, 2017, 416(1-2):259-269.

Yan Y , Song X , Li Z , et al. Elevated levels of granzyme B correlated with miR-874-3p downregulation in patients with acute myocardial infarction[J]. Biomarkers in Medicine, 2017, 11(9):761-767.

Wang J , Yan Y , Song D , et al. The association of plasma miR-155 and VCAM-1 levels with coronary collateral circulation[J]. Biomarkers in Medicine, 2017, 11(2):125-131.

Zhang Y , Weng S , Yin J , et al. Vitamin K2 promotes mesenchymal stem cell differentiation by inhibiting miR?133a expression[J]. Molecular Medicine Reports, 2017, 15(5):2473-2480.