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Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

DOPA Decarboxylase (DDC) Human siRNA Oligo Duplex (Locus ID 1644)

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Katalog-Nummer SR301162

Size : 1kit

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DOPA Decarboxylase (DDC) Human siRNA Oligo Duplex (Locus ID 1644)

SKU

SR301162

DDC (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Stability One year from date of shipment when stored at -20°C. # of transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). Note Single siRNA duplex (10nmol) can be ordered. Reference Data RefSeq NM_000790, NM_001082971, NM_001242886, NM_001242887, NM_001242888, NM_001242889, NM_001242890 UniProt ID P20711 Synonyms AADC Summary The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011] Performance Guaranteed OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at tech@biotrend.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).