Donkey normal serum
Katalog-Nummer AS101564
Size : 10ml
Marke : Agrisera
Contact local distributor :
Telefonnummer : +1 850 650 7790
Donkey normal serum (10 ml)
Product no: AS10 1564
-
This product doesn't have any reviews.
| Host: | Donkey |
| Purity: | Serum |
| Format: | Lyophilized |
| Quantity: | 10 ml |
| Reconstitution: | For reconstitution add 11.0 ml of sterile water |
| Storage: | Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 ºC. For storage at 2-8°C, add a preservative to prevent growth of bacteria. Rehydrate with 11.0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates. |
| Tested applications: | ELISA (ELISA). Immunofluorescence (IF), Western blot (WB) |
| Additional information: | Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial. For a Western Blot 10-20 ml of blocking agent is needed, aliquote and freeze the rest of the unused blocking buffer, because there are no preservatives in the serum. Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.As a blocking agent, this product is used to block non specific interaction with conjugates of donkey primary or secondary antibodies. |
| Background: | Normal donkey serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications. |
Sie könnten auch an folgenden Produkten interessiert sein:
Katalog-Nummer
Beschreibung
Cond.
Preis zzgl. MwSt.