• SKU 715-035-150
    Host Species

    Donkey
    IgG Form

    IgG whole molecule
    Species Reactivity

    Mouse
    Specificity

    IgG (H+L)
    Isotype

    Ig
    Clonality (Mono-/Polyclonal)

    polyclonal
    Application

    ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections), Western Blot
    Conjugation

    Horseradish Peroxidase (HRP)
    No Cross-reactivity (MinX) with

    Bovine, Chicken, Goat, Guinea Pig, Hamster syrian, Horse, Human, Rabbit, Sheep
    Dilution

    ELISA 1:5,000 – 1:100,000, Histo-/Cytochemistry 1:500 – 1:5,000, Western Blot (ECL): 1:10,000 – 1:200,000, Western Blot (non-ECL): 1:5,000 – 1:100,000
    Format

    15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS (pH 7.6), lyophilisate
    Intended Use

    for Research Use Only
    Product line / Topic

    Jackson ImmunoResearch
    Manufacturer / Brand

    Jackson ImmunoResearch
  • Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum proteins, but it may cross-react with immunoglobulins from other species.

    Conjugate

    Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.