Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 
> DEPC

DEPC

Drucken Drucken
Melden Sie sich an
Mindestbestell 2

Katalog-Nummer DB0154-25ml

Size : 25ml

Marke : Bio Basic

Weitere Informationen anfordern

Contact local distributor :


Telefonnummer : +1 850 650 7790

DEPC (Diethyl pyrocarbonate)
CAS: [1609-47-8]
Storage: 2 to 8C
Product Description: DEPC

Molecular formula: C6H10O5

Diethyl Pyrocarbonate (DEPC) is sensitive to moisture and to pH; it decomposes to ethanol and carbon dioxide in aqueous solution. It decomposes at 155°C. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.

DEPC is effective as a nuclease inhibitor. It reacts with many enzymes containing -NH, -SH or - OH groups in their active sites. Typically a 0.1% solution is used to inactive RNase (one milliliter of DEPC is added to 1 liter of water).

The DEPC will not immediately dissolve, as evidenced by the appearance of globules. The mixture should be stirred until the globules disappear.

The DEPC hydrolyzes over time to form ethanol and carbon dioxide. At this time the solution may be autoclaved to destroy the DEPC. DEPC has been used as a gentle esterifying agent and preservative.

DEPC acts as a condensing agent between lysine e-amine groups and the carboxyl groups of aspartic or glutamic acid. It modifies purine residues in RNA by carboxyethylation of histidyl residues. It is considered sufficient to autoclave 0.1% DEPC solutions for 15 minutes per liter. A method to assay for DEPC uses 5-thio-2- nitrobenzoate.

For general information and background, see references
Total Product Size:
DB0154 (5ml)
DB0154 (25ml)
DB0154 (100ml)
Number of Containers: 1
Refrigeration Requirements: Refrigerator
Shipping Conditions: ICE
UNSPSC Code: 12352200
UNSPSC Category: Inhibitors
Documents

Sie könnten auch an folgenden Produkten interessiert sein:



Katalog-Nummer
Beschreibung
Cond.
Preis zzgl. MwSt.
331-12036-1
SYBR Safe DNA Gel Stain
 500ul 
0100-20
DAPI Fluoromount-G®
 20mL 
0100-35
Fluoromount-G® Anti-Fade
 20mL