Immunofluorescence. Cytological material. Flow Cytometry: 1/5-1/10. Immunohistochemistry on Frozen Sections: 1/10. Immunohistochemistry on Paraffin Sections: 1/10. Incubation Time: 1 h at 37°C.
Reactivity
Bovine, Canine, Hamster, Human, Mouse, Porcine, Rat, Sheep, Trout
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Human keratin K18 from a HeLa cytoskeletal preparation
Specificity
Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tested Tumors Specifically Detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide Reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reacts with HeLa and MCF-7 (cell lines tested so far).
Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
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