Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker)(rKRT10/844), 1mg/mL
Katalog-Nummer BNUM1989-50
Size : 50uL
Marke : Biotium
Contact local distributor :
Telefonnummer : +1 850 650 7790
| Antibody number | #1989 |
|---|---|
| Antibody reactivity (target) | Cytokeratin 10, KRT10 |
| Antibody type | Primary |
| Host species | Mouse |
| Clonality | Recombinant Monoclonal |
| Clone | rKRT10/844 |
| Isotype | IgG1, kappa |
| Molecular weight | 56.5 kDa |
| Synonyms | BCIE; BIE; EHK; Keratin Type I Cytoskeletal 10; KRT10 |
| Human gene symbol | KRT10 |
| Entrez gene ID | 3858 |
| SwissProt | P13645 |
| Unigene | 99936 |
| Immunogen | Recombinant full-length human KRT10 protein. |
| Verified antibody applications | IHC (FFPE) (verified) |
| Antibody target cellular localization | Cytoskeleton |
| Species reactivity | Human, Mouse |
| Positive control | A431, HeLa, MCF7 cells. Esophagus or tonsil. |
| Shipping condition | Room temperature |
| Storage Conditions | Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C |
| Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
| Regulatory status | For research use only (RUO) |
| Antibody/conjugate formulation | Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide |
| Antibody research areas | Cancer, Cytoskeleton |
| Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
| Cell/tissue expression | Epidermal cells |
| Tumor expression | Squamous cell carcinoma |
Product Description
This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the
CF® Dye Brochure
for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.info@biotrend.com
to inquire about stock status and lead times before placing your order.Catalog number key for antibody number 1989, Anti-Cytokeratin 10 (rKRT10/844)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
|---|---|---|---|---|---|
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified, BSA-free | N/A | N/A | N/A |