Cholesterol Oxidase Goat Polyclonal Antibody
Katalog-Nummer R1075BS
Size : 100ug
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Cholesterol Oxidase Goat Polyclonal Antibody
| Product Data | |
| Application | ELISA, IP, WB |
|---|---|
| Recommended Dilution | Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of Cholesterol oxidase [microorganism] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested. |
| Reactivity | Bacteria |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Cholesterol oxidase from Streptomyces |
| Specificity | This product detects Cholesterol oxidase (microorganism). Cross reactivity against Cholesterol oxidase from other sources is unknown. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified Cholesterol oxidase [microorganism]. |
| Buffer | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/m BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium azide |
| Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
| Concentration | lot specific |
| Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
| Conjugation | Biotin |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Background | Cholesterol oxidases exist as both type I and type II oxidases and are implicated in bacterial pathogenesis. In addition, they are important as clinical reagents, potential larvicides, and tools in cell biology. |
| Synonyms | Cholesterol oxidase, CHOD, EC 1.1.3.6 |
| Reference Data | |