CD62P (FLJ45155, Granule Membrane Protein 140kDa, GMP140, Granulocyte Membrane Protein, GMRP, GRMP, Leukocyte Endothelial Cell Adhesion Molecule 3, LECAM3, P-Selectin, PSEL, Platelet Activation Dependent Granule External Membrane Protein, PADGEM, Selectin P (Granule Membrane Protein 140kDa Antigen CD62), SELP) (PE)

Katalog-Nummer C2415-54B3-100T

Size : 100Tests

Marke : US Biological

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C2415-54B3 CD62P (FLJ45155, Granule Membrane Protein 140kDa, GMP140, Granulocyte Membrane Protein, GMRP, GRMP, Leukocyte Endothelial Cell Adhesion Molecule 3, LECAM3, P-Selectin, PSEL, Platelet Activation Dependent Granule External Membrane Protein, PADGEM, Selectin P (Granule Membrane Protein 140kDa Antigen CD62), SELP) (PE)

Clone Type
Polyclonal
Host
mouse
Source
human
Isotype
IgG1
Grade
Affinity Purified
Applications
FC
Crossreactivity
Gt Hu Rt Sh
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CD62P is a 140kD antigen expressed on activated platelets and also on endothelial cells both of blood and lymph vessels. CD62P (also known as PADGEM or GMP140) is a component of the alpha granule and is translocated to the plasma membrane upon activation. C2415-54B3 is reported to inhibit P-selectin-dependent adhesion between HL-60 cells and P-selectinm transfected COS cells. ||Applications:|Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at 4°C in the dark. DO NOT FREEZE! Stable for 12 months after receipt at 4°C. Reconstitute with sterile ddH2O. Store at 4°C. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 10B1879 (Psel.KO.2.12)|Host: mouse|Source: human|Concentration: Not determined|Form: Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide, 5% sucrose. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.|Purity: Purified by Protein A affinity chromatography.|Immunogen: CD62P transfected 300.19 cells.|Specificity: Recognizes human CD62P. Species crossreactivity: goat, rat, sheep||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
CD62P transfected 300.19 cells.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide, 5% sucrose. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD62P. Species crossreactivity: goat, rat, sheep
References
1. Massaguer, A. et al. (2000) Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P). Tissue antigens 56: 117-128, 2. Massaguer, A. et al. (2003) Characterization of platelet and soluble-porcine P-selectin (CD62P). Vet. Immunol. Immunopathol. 96: 169-18, 3. 3. Massaguer, A. et al. (2002) Reactivity of CD62P workshop mAbs with resting and activated platelets from different animal species. In: Leucocyte Typing VII. Edited by Mason, D. et al. Oxford University Press, pp 342-343.