Flow Cytometry analysis of Human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Antibody Host
Mouse
Isotype
IgG2a
Clonality
Monoclonal
Immunogen
Human tonsil.
Specificity
This antibody reacts with Human CD52 (CAMPATH-1), a 21-28 kDa glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract.
Buffer
PBS Label: APC State: Liquid purified IgG fraction Stabilizer: 0.2% (w/v) high-grade protease free BSA Preservative: 15 mM Sodium Azide Label: Conjugated with cross-linked Allophycocyanin under optimum conditions
Purification
Size-Exclusion Chromatography
Conjugation
APC
Storage
Store undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.
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