CD5 (CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5) (PE)

Recognizes a 67kDa transmembrane protein, which is identified as CD5 (HLDA I; WS Code T 29. HLDA III; WS Code T 530). The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms.||Applications: |Suitable for use in Immunofluorescence, ELISA, Flow Cytometry (Not Tested), Immunoprecipitation, Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Flow Cytometry (Not Tested): 0.5-1ug/million cells|Immunofluorescence: 0.5-1ug/ml|Immunoprecipitation: 0.5-1ug/500ug protein lysate|Immunohistochemistry: Frozen: 0.5-1.0ug/ml for 30 minutes at RT|Optimal dilutions to be determined by the researcher.||Positive Control:|293T, Ramos, or MOLT-4 Cells. Tonsil.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.