CBP, p300 (CREB Binding Protein, Histone Acetyl Transferase, RTS, RSTS, Rubinstein-Taybi Syndrome) (BSA & Azide Free) (HRP)

Katalog-Nummer C2098-03X-HRP-100ul

Size : 100ul

Marke : US Biological

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C2098-03X-HRP CBP, p300 (CREB Binding Protein, Histone Acetyl Transferase, RTS, RSTS, Rubinstein-Taybi Syndrome) (BSA & Azide Free) (HRP) discontinued

Clone Type
Polyclonal
Host
mouse
Source
human
Isotype
IgG2b
Grade
Affinity Purified
Applications
IF IHC
Crossreactivity
Hu Mk Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

p300 is a transcriptional adapter protein which is characterized by three cysteine-and histidine-rich regions and its C-terminus specifically binds the adenovirus E1A protein. p300, and p300-associated proteins, are components of TATAbinding protein (TBP) complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265kD nuclear protein designated CBP (for CREB-binding protein). CBP and p300 are homologous to each other.||Applications:|Suitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot. Other applications have not been tested.||Recommended Dilution: |Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.|Optimal dilutions to be determined by the researcher||Positive Control: Tonsil||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2b|Clone No: 3F310|Host: mouse|Source: human|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein A affinity chromatography.|Immunogen: E1A affinity purified p300 protein. Cellular Localization; Nuclear. MW of Antigen; 300kD. Epitope; aa2071-2091.|Specificity: Recognizes human p300 and CBP(1). p300 and CBP differ by three noncontiguous residues within the 21aa epitope region. A difference that does not detectably affect the reactivity of C2098-03. Species Crossreactivity: monkey, mink, mouse and rat.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
E1A affinity purified p300 protein. Cellular Localization; Nuclear. MW of Antigen; 300kD. Epitope; aa2071-2091.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human p300 and CBP(1). p300 and CBP differ by three noncontiguous residues within the 21aa epitope region. A difference that does not detectably affect the reactivity of C2098-03. Species Crossreactivity: monkey, mink, mouse and rat.
References
1. Dallas PB, et. al. Journal of Virology, 1997, 71(2):1726-31. 2. Dallas PB, et. al. Hybridoma, 1997, 16:273-5.