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> BODIPY FL prazosin [175799-93-6]

BODIPY FL prazosin [175799-93-6]

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Katalog-Nummer HY-D1606-10mg

Size : 10mg

Marke : MedChemExpress

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BODIPY FL prazosin is a fluorescent α1-adrenergic antagonist with Ki values of 14.5, 43.3 nM for α1a-AR and α1b-AR, respectively. BODIPY FL prazosin also is a fluorescent ligand with the excitation and emission wavelengths are 485 and 535 nm, respectively. BODIPY FL prazosin can be used for study the differences in the subcellular localization of α1-adrenoceptor subtypes.

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BODIPY FL prazosin Chemische Struktur

BODIPY FL prazosin Chemische Struktur

CAS. Nr. : 175799-93-6

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Alle Adrenergic Receptor Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen
α adrenergic receptor β adrenergic receptor
Beschreibung

BODIPY FL prazosin is a fluorescent α1-adrenergic antagonist with Ki values of 14.5, 43.3 nM for α1a-AR and α1b-AR, respectively. BODIPY FL prazosin also is a fluorescent ligand with the excitation and emission wavelengths are 485 and 535 nm, respectively. BODIPY FL prazosin can be used for study the differences in the subcellular localization of α1-adrenoceptor subtypes[1][2][3].

IC50 & Target[1]

α1A-adrenergic receptor

14.5 nM (Ki)

α1B-adrenergic receptor

43.3 nM (Ki)

In Vitro

BODIPY FL prazosin (10 nM; 30 min at room temperature in 100 μl; COS-7 cells) shows Affinity of various α1-AR ligands with Ki values of 14.5, 43.3 nM for α1a-AR and α1b-AR, respectively[1].
BODIPY FL prazosin (100 nM, 30 min) can be used as molecular probe for the Visualization of the non-adrenoceptor binding site of α1-adrenergic drugs in erythroleukemia cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BODIPY FL prazosin Related Antibodies
Molekulargewicht

563.41

Formel

C28H32BF2N7O3
CAS. Nr.

175799-93-6
Appearance

Solid

Color

Yellow to orange

Emission (Em)

510
Excitation (Ex)

502
SMILES

O=C(CCC1=CC=C2C=C3C(C)=CC(C)=73[B+3]([F-])([N-]21)[F-])N4CCN(CC4)C5=NC6=C(C=C(C(OC)=C6)OC)C(N)=N5
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 12.2 mg/mL (21.65 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7749 mL 8.8745 mL 17.7491 mL
5 mM 0.3550 mL 1.7749 mL 3.5498 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Reinheit & Dokumentation

Purity: 99.71%

SDS (252 KB)

COA (251 KB) HNMR (280 KB) RP-HPLC (234 KB) MS (68 KB)

Handling Instructions (2659 KB)
Verweise

  • [1]. Sugawara T, et al. Differences in the subcellular localization of alpha1-adrenoceptor subtypes can affect the subtype selectivity of drugs in a study with the fluorescent ligand BODIPY FL-prazosin. Life Sci. 2002 Mar 22;70(18):2113-24.  [Content Brief]

    [2]. Cerveny L, et al. Lack of interactions between breast cancer resistance protein (bcrp/abcg2) and selected antiepileptic agents. Epilepsia. 2006 Mar;47(3):461-8.  [Content Brief]

    [3]. Fuchs R, et al. α1-adrenergic drugs exhibit affinity to a thapsigargin-sensitive binding site and interfere with the intracellular Ca2+ homeostasis in human erythroleukemia cells. Exp Cell Res. 2011 Dec 10;317(20):2969-80.  [Content Brief]

  • [1]. Sugawara T, et al. Differences in the subcellular localization of alpha1-adrenoceptor subtypes can affect the subtype selectivity of drugs in a study with the fluorescent ligand BODIPY FL-prazosin. Life Sci. 2002 Mar 22;70(18):2113-24.

    [2]. Cerveny L, et al. Lack of interactions between breast cancer resistance protein (bcrp/abcg2) and selected antiepileptic agents. Epilepsia. 2006 Mar;47(3):461-8.

    [3]. Fuchs R, et al. α1-adrenergic drugs exhibit affinity to a thapsigargin-sensitive binding site and interfere with the intracellular Ca2+ homeostasis in human erythroleukemia cells. Exp Cell Res. 2011 Dec 10;317(20):2969-80.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7749 mL 8.8745 mL 17.7491 mL 44.3727 mL
5 mM 0.3550 mL 1.7749 mL 3.5498 mL 8.8745 mL
10 mM 0.1775 mL 0.8875 mL 1.7749 mL 4.4373 mL
15 mM 0.1183 mL 0.5916 mL 1.1833 mL 2.9582 mL
20 mM 0.0887 mL 0.4437 mL 0.8875 mL 2.2186 mL
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BODIPY FL prazosin Related Classifications

  • Metabolic Disease
  • GPCR/G Protein Neuronal Signaling
  • Adrenergic Receptor
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BODIPY FL prazosin175799-93-6Adrenergic ReceptorBeta ReceptorCOS-7 cellsα1a-ARα1b-ARMEF3.8-BCRP cellsmolecular probeerythroleukemia cellsInhibitorinhibitorinhibit