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> beta Amyloid 1-42 Antibody

beta Amyloid 1-42 Antibody

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Katalog-Nummer bs-0076R-20ul

Size : 20ul

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bs-0076R [Primary Antibody]
beta Amyloid 1-42 Antibody
www.biossusa.com
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800.501.7654 [DOMESTIC]
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Host: Rabbit

Target Protein: beta Amyloid 1-42

Immunogen Range: 35-42/42


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 351

Swiss Prot: P05067

Source: KLH conjugated synthetic peptide derived from human beta-Amyloid(35-42)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Beta-amyloid 42 is a more effective reductant than beta-amyloid 40. Beta-amyloid peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
IP(1-2ug)

Predicted Molecular Weight: 4.3/75


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Pi, Jiangli, et al. "A sandwich immunoassay for detection of Aβ 1-42 based on quantum dots." Talanta (2015): TALD1501332.Read more>>
  • Luo et al. A self-destructive nanosweeper that captures and clears amyloid β-peptides. (2018) Nat.Commun. 9:1802Read more>>
  • Fan Y et al. Up-regulation of TREM2 accelerates the reduction of amyloid deposits and promotes neuronal regeneration in the hippocampus of amyloid beta1-42 injected mice. J Chem Neuroanat. 2019 Feb 18;97:71-79. Read more>>
  • Wen, Jinhua. et al. Uptake of A by OATPs might be a new pathophysiological mechanism of Alzheimer disease. Bmc Neurosci. 2021 Dec;22(1):1-9Read more>>
  • Li Xuewang. et al. Inhibition of NLRP1 inflammasome improves autophagy dysfunction and A disposition in APP/PS1 mice. BEHAV BRAIN FUNCT. 2023 Dec;19(1):1-19Read more>>
  • nal Glin ?zdamar. et al. The beneficial effects of vortioxetine on BDNF, CREB, S100B, amyloid, and glutamate NR2b receptors in chronic unpredictable mild stress model of depression. PSYCHOPHARMACOLOGY. 2023 Aug;:1-15Read more>>
VALIDATION IMAGES

Mouse spleen cells probed with beta Amyloid 1-42 Antibody, unconjugated (bs-0076R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)