BCMA IgG2a Fc, Fusion Protein (B Cell Maturation Protein)

The human B cell activating factor (BAFF) and APRIL (a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily. They are expressed by non-B cells and are down-regulated by mitogenic stimulation. BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells. Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells. Recently a third receptor for BAFF (BAFF-R) was described. BAFF and BAFF-R knockout mice have a reduced number of mature B cells in the periphery. However, TACI and BCMA knockouts do not share this phenotype, suggesting that BAFF-R may be the primary receptor for BAFF in mice. Cell surface BAFF can be cleaved to form a soluble trimeric molecule. Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren’s Syndrome. BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit binding of this molecule to receptors on Raji cells. |BCMA IgG2a Fc, Fusion Protein is a soluble molecule consisting of the extracellular domain (54 amino acids) of human BCMA fused to murine IgG2a Fc (232 amino acids).||Applications: |Suitable for use in ELISA. BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit this molecule's abiltiy to bind to receptors on Raji cells. Other applications not tested.| |Recommended Dilutions:|Optimal dilutions to be determined by the researcher. ||Binding Inhibition of Raji Cell Receptors:|B0807-50B was tested for its ability to block the binding of B0003-67EX to receptors on Raji cells. 5x10e5 human Raji cells were washed and incubated for 45 min. on ice with 80ul of C2432-01H at 1ug/ml. Cells were washed 2 times and incubated with C2259-11D. Cells were then washed 3 times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared with a buffer control. This binding was 88% inhibited when B0003-67EX was pre-incubated with 50ug/ml B0807-50B.||Storage and Stability:|May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.