Sample Type:|Cell culture||Reagent Name: |7-AAD, CFSE||Target:|Cell-mediated cytotoxicity||Excitation/Emission:|7-AAD–546nm/647nm; CFSE–492nm/520nm||Method of Analysis:|Flow cytometry||Intended Use:|Basic Cytotoxicity Test Assay is a single-tube, dual-color assay for determining cytotoxicity by flow cytometry. The BioAssay™ employs a green fluorescent cellular stain, CFSE, to label target cells and the red live/dead viability dye, 7-AAD, to identify the dead cells present in the cytotoxicity BioAssay™ samples. Analyze your results using a flow cytometer.||Test Principle:|Cytolytic activity is an important process for eliminating intracellular pathogens and cancer cells. Basic Cytotoxicity Assay allows you to assess cytolytic activity in cell culture.||The Basic Cytotoxicity BioAssay™ includes two fluorescent reagents: CFSE and 7-AAD. CFSE, a green fluorescing cellular stain, is utilized to identify the target cell population. The unstained effector cells are added and incubated with the target cells. 7-AAD, a red fluorescing live/dead stain, is then added to stain all necrotic cells red by binding to the DNA of membrane-compromised cells. The target cell and effector cell populations can then be easily distinguished, and flow cytometry easily identifies live and necrotic cells.||Kit Components:|125 tests:|CFSE target cell stain, 1 vial|7-AAD vital stain, 1 vial|10X Assay Buffer, 1x30mL||250 tests:|CFSE target cell stain, 1 vial|7-AAD vital stain, 2 vials|10X Assay Buffer, 1x60mL||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.