ATP BioAssay™ Kit

Adenosine triphosphate (ATP) is a nucleoside triphosphate formed exclusively in the mitochondria. It is a high-energy molecule described as the energy currency in all living systems. The chemical energy contained in the phosphate bond of ATP drives most cellular processes. Genetic disorders such as Leber hereditary optic neuropathy, Leigh syndrome, neuropathy, ataxia, and retinitis pigmentosa affect the generation of ATP in the mitochondria.||Sample Type:|Erythrocyte, tissue||Intended Use:|This kit can be used for detection of ATP activity in erythrocyte or tissue samples.||Kit Components (see Protocol for final kit quantities):|Reagent 1,|Substrate I, powderx1 vial||Reagent 2,|Substrate II, 20 mLx1 vial||Reagent 3,|Accelerant, powderx2 vial|Accelerant diluent, 760 uLx2 vial||Reagent 4,|Precipitant, 5.5 mLx1 vial||Reagent 5,|Developer A solution, 7 mLx4 vial|Developer B solution, 6 mLx4 vial||Reagent 6,|Stop Solution, 50 mLx1 vial||Reagent 7,|ATP Standard, powderx2 vial||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.