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> Arginase 1 (Hepatocellular Carcinoma Marker) (ARG1/1125) , 0.2mg/mL

Arginase 1 (Hepatocellular Carcinoma Marker) (ARG1/1125) , 0.2mg/mL

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Katalog-Nummer BNUB1125-500

Size : 500uL

Marke : Biotium

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Telefonnummer : +1 850 650 7790

Formalin-fixed paraffin-embedded human Hepatocellular Carcinoma stained with ARG 1 Mouse Monoclonal Antibody (ARG1/1125).
Western Blot Analysis A) Recombinant ARG1 Protein Fragment (B) humanLiver lysate Using ARG1 Mouse Monoclonal Antibody (ARG1/1125).
SDS-PAGE Analysis Purified ARG1Mouse Monoclonal Antibody (ARG1/1125). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19000 full-length human proteins using Arginase-1 Mouse Monoclonal Antibody (ARG1/1125). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target if the MAb has an S-score of at least 2.5. For example if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14 then the S-score for the binding of that MAb to protein X is equal to 29.
research-areas

Metabolism
Antibody number

#1125
Antibody reactivity (target)

Arginase 1
Antibody type

Primary
Host species

Mouse
Clonality

Monoclonal
Clone

ARG1/1125
Isotype

IgG3, kappa
Molecular weight

35-38 kDa
Synonyms

Arginase 1; ARG1; liver-type arginase; type I arginase
Human gene symbol

ARG1
Entrez gene ID

383
SwissProt

P05089
Unigene

440934
Immunogen

Recombinant human ARG1 protein fragment (around aa11-97) (exact sequence is proprietary)
Verified antibody applications

IHC (FFPE) (verified), WB (verified)
Antibody target cellular localization

Cytoplasmic
Species reactivity

Human
Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistology (formalin): 2-4 ug/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Western blotting 1-2 ug/mL, Optimal dilution for a specific application should be determined by user
Positive control

293Tcells. Hepatocellular Carcinoma (HCC).
Shipping condition

Room temperature
Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status

For research use only (RUO)
Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Validated in protein array

Monospecific
Antibody research areas

Cancer, Metabolism
Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Cell/tissue expression

Liver
Protocols

PI Protocols for Antibody Based Detection

SDS

SDS Primary Antibodies

Download Datasheet

Product Description

This antibody recognizes a protein of 35-38 kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibodies to Arginase 1 label hepatocytes in normal tissues and granulocytes in peripheral blood. Arginase 1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the

CF® Dye Brochure

for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email

info@biotrend.com

to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 1125, Anti-Arginase 1 (ARG1/1125) 

Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405

CF®405S Features
BNC88 CF®488A 490/515 488 GFP, FITC

CF®488A Features
BNC68 CF®568 562/583 532, 561 RFP, TRITC

CF®568 Features
BNC94 CF®594 593/614 561 Texas Red®

CF®594 Features
BNC40 CF®640R 642/662 633-640 Cy®5

CF®640R Features
BNC47 CF®647 650/665 633-640 Cy®5

CF®647 Features
BNC74 CF®740 742/767 633-685 775/50

CF®740 Features
BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free		 N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.