Anti-Phospho-STK11-Ser428 antibody (384-433 aa)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:40000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	STK11
Gene ID:	6794
Uniprot ID:	STK11_HUMAN
Immunogen Region:	384-433 aa
Specificity:	Phospho-LKB1 (S428) Polyclonal Antibody detects endogenous levels of LKB1 protein only when phosphorylated at S428.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human LKB1 around the phosphorylation site of Ser428 at the amino acid range 384-433

Post Translational Modifications	Phosphorylated by ATM at Thr-363 following ionizing radiation (IR). Phosphorylation at Ser-428 by RPS6KA1 and/or some PKA is required to inhibit cell growth. Phosphorylation at Ser-428 is also required during neuronal polarization to mediate phosphorylation of BRSK1 and BRSK2. Phosphorylation by PKC/PRKCZ at Ser-399 in isoform 2 promotes metformin (or peroxynitrite)-induced nuclear export of STK11 and activation of AMPK. UV radiation-induced phosphorylation at Thr-363 mediates CDKN1A degradation. Acetylated. Deacetylation at Lys-48 enhances cytoplasmic localization and kinase activity in vitro.
Function	Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53.the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair. Isoform 2: Has a role in spermiogenesis.
Protein Name	Serine/Threonine-Protein Kinase Stk11 Liver Kinase B1 Lkb1 Hlkb1 Renal Carcinoma Antigen Ny-Ren-19
Database Links	Reactome: R-HSA-163680 Reactome: R-HSA-380972 Reactome: R-HSA-6804756 Reactome: R-HSA-9614657
Cellular Localisation	Nucleus Cytoplasm Membrane Mitochondrion A Small Fraction Localizes At Membranes Relocates To The Cytoplasm When Bound To Strad And Cab39/Mo25 (Cab39/Mo25alpha Or Cab39l/Mo25beta) Translocates To The Mitochondrion During Apoptosis Pten Promotes Cytoplasmic Localization Isoform 2: Nucleus Predominantly Nuclear But Translocates To The Cytoplasm In Response To Metformin Or Peroxynitrite Treatment
Alternative Antibody Names	Anti-Serine/Threonine-Protein Kinase Stk11 antibody Anti-Liver Kinase B1 antibody Anti-Lkb1 antibody Anti-Hlkb1 antibody Anti-Renal Carcinoma Antigen Ny-Ren-19 antibody Anti-STK11 antibody Anti-LKB1 antibody Anti-PJS antibody

Information sourced from Uniprot.org

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