Western blot of nonreduced (-) and reduced (+) mouse IgG1κ and IgG1λ (20 ng/lane), using 0.2 µg/mL of RevMAb Clone RM103. This antibody reacts to nonreduced IgG1κ (~150 kDa), and slightly reacts to reduced κ light chain (~25 kDa). ELISA of mouse immunoglobulins shows RM103 reacts to the kappa light chain of mouse immuno-globulins. No cross reactivity with the lamda light chain, human IgG (κ+λ), rat IgG (κ+λ), or goat IgG (κ+λ). The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. A titer ELISA of mouse IgG1κ. The plate was coated with different amounts of mouse IgG1κ. A serial dilution of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Product Details Product Name Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody, Clone RM103 Product Description Rabbit monoclonal to Mouse Ig Kappa Light Chain Catalog No. 31-1007-00 Clone Name RM103 Product Data Sheet Anti-Mouse Ig Kappa Rabbit Monoclonal Antibody Data Sheet RM103 Specificity This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. Immunogen Mouse IgG Application ELISA
Western Blot (WB) (nonreduced) Species Reactivity Mouse Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 0.005 µg/mL - 0.2 µg/mL;
Western Blot (WB): 0.1 µg/mL - 0.5 µg/mL. Keywords . Product Images
Anti-Mouse Ig Kappa Light Chain rabbit monoclonal antibody [RM103]
![Anti-Mouse Ig Kappa Light Chain rabbit monoclonal antibody [RM103]](https://www.biotrend.com/upload/-img-lapin1.png)
Katalog-Nummer 31-1007-00
Size : 100ug
Marke : RevMab Biosciences
Contact local distributor :
Telefonnummer : +1 850 650 7790
Rabbit monoclonal to Mouse Ig Kappa Light Chain
100 µg
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