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SKU DIA-KK3 Specificity CD73
Species Reactivity Human
Immunogen Recombinant peptide of human CD73
Host Species Mouse
Isotype IgG2a/k
Clone KK3
Clonality (Mono-/Polyclonal) monoclonal
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections), Western Blot
Conjugation unconjugated
Dilution Immunohistochemistry (IHC): 1:100 – 1:200
Format 0.05% NaN3, 2% BSA, in PBS (pH 7.4), lyophilisate, purified antibody (from culture supernatant)
Product line / Topic Immuno-Oncology, Tumor Marker / Biology
Intended Use for Research Use Only
Temperature - Storage 2-8°C
Temperature - Transport at room temperature
Search Code DIAKK3, KK3, IHC, FFPE, Paraffin
Manufacturer / Brand ONCOdianova
Uniprot_ID P21589
Gene_ID 4907
Alias 5-NT, 5-Nucleotidase, 5'-Nucleotidase, 5'-Nucleotidase, Ecto, CD73, E5-NT, E5NT, EN, ENT, NT5-E, Nt5e
- Clone KK3 has been developed and validated specifically for the immunohistochemical (IHC) detection of CD73 in routine FFPE human tissue specimen. Given the broad expression of ectonucleotidases and adenosine receptors, immunohisto-chemical (IHC) application of monoclonal antibody KK3 may help to develop a better understanding of cell- and tissue- specific roles.
CD73 and CD39 are cell surface enzymes that catabolize the breakdown of extracellular ATP into adenosine. As ectonucleo-sidases they play important roles in maintaining tissue and immune homeostasis through interfering with the extracellular purigeneric pathway. Since several labs independently have demonstrated an immunosuppressive role of CD73-adenosine in cancer, the CD73-adenosine axis has emerged as one of the most promising therapeutic targets in immuno-oncology.
Adenosine triphosphate (ATP) is the major source of energy for the cell. Malignant cells can release high levels of ATP (e.g. after damage by radiotherapy or chemotherapy). Extracellular ATP provokes inflammation by driving "purinergic signals&rsquo and plays a significant role in promoting anti-tumor responses. Many tumors express the ectonucleotidases CD39 and CD73 to scarve such proinflammatory mediators and generate immunosuppressive adenosine nucleosides.
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Anti-CD73 (Hu) from Mouse (KK3)
Katalog-Nummer DIA-KK3
Size : 500µl