• SKU DIA-KK3
    Specificity

    CD73

    Species Reactivity

    Human

    Immunogen

    Recombinant peptide of human CD73

    Host Species

    Mouse

    Isotype

    IgG2a/k

    Clone

    KK3

    Clonality (Mono-/Polyclonal)

    monoclonal

    Application

    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections), Western Blot

    Conjugation

    unconjugated

    Dilution

    Immunohistochemistry (IHC): 1:100 – 1:200

    Format

    0.05% NaN3, 2% BSA, in PBS (pH 7.4), lyophilisate, purified antibody (from culture supernatant)

    Product line / Topic

    Immuno-Oncology, Tumor Marker / Biology

    Intended Use

    for Research Use Only

    Temperature - Storage

    2-8°C

    Temperature - Transport

    at room temperature

    Search Code

    DIAKK3, KK3, IHC, FFPE, Paraffin

    Manufacturer / Brand

    ONCOdianova

    Uniprot_ID

    P21589

    Gene_ID

    4907

    Alias

    5-NT, 5-Nucleotidase, 5'-Nucleotidase, 5'-Nucleotidase, Ecto, CD73, E5-NT, E5NT, EN, ENT, NT5-E, Nt5e

  • Clone KK3 has been developed and validated specifically for the immunohistochemical (IHC) detection of CD73 in routine FFPE human tissue specimen. Given the broad expression of ectonucleotidases and adenosine receptors, immunohisto-chemical (IHC) application of monoclonal antibody KK3 may help to develop a better understanding of cell- and tissue- specific roles.

    CD73 and CD39 are cell surface enzymes that catabolize the breakdown of extracellular ATP into adenosine. As ectonucleo-sidases they play important roles in maintaining tissue and immune homeostasis through interfering with the extracellular purigeneric pathway. Since several labs independently have demonstrated an immunosuppressive role of CD73-adenosine in cancer, the CD73-adenosine axis has emerged as one of the most promising therapeutic targets in immuno-oncology.

    Adenosine triphosphate (ATP) is the major source of energy for the cell. Malignant cells can release high levels of ATP (e.g. after damage by radiotherapy or chemotherapy). Extracellular ATP provokes inflammation by driving "purinergic signals&rsquo and plays a significant role in promoting anti-tumor responses. Many tumors express the ectonucleotidases CD39 and CD73 to scarve such proinflammatory mediators and generate immunosuppressive adenosine nucleosides.

  • CD73 immunohistochemistry in a tonsil.
    CD73 immunohistochemistry in a tonsil.
    Strong homogeneous CD73 immunostaining in a cholangiocellular carcinoma of the liver.
    Strong homogeneous CD73 immunostaining in a cholangiocellular carcinoma of the liver.
    Membranous CD73 immunostaining in an adenocarcinoma of the lung shows apical predominance.
    Membranous CD73 immunostaining in an adenocarcinoma of the lung shows apical predominance.
    Prominent membranous CD73 positivity in a malignant melanoma.
    Prominent membranous CD73 positivity in a malignant melanoma.