Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 
> Androgen Receptor(DHTR/882), RPE conjugate, 0.1mg/mL

Androgen Receptor(DHTR/882), RPE conjugate, 0.1mg/mL

Drucken Drucken
Melden Sie sich an

Katalog-Nummer BNCR0882-250

Size : 250uL

Marke : Biotium

Weitere Informationen anfordern

Contact local distributor :


Telefonnummer : +1 850 650 7790

Androgen Receptor Monoclonal Mouse Antibody (DHTR/882)

Product Attributes

Antibody number

#0882
research-areas

Cancer, Endocrinology, Signal transduction
Antibody reactivity (target)

Androgen Receptor
Antibody type

Primary
Host species

Mouse
Clonality

Monoclonal
Clone

DHTR/882
Isotype

IgG1, kappa
Molecular weight

~110 kDa
Synonyms

Androgen Receptor; AR; Dihydrotestosterone Receptor; Nuclear Receptor Subfamily 3 Group C Member 4
Human gene symbol

AR
Entrez gene ID

367
SwissProt

P10275
Unigene

496240
Immunogen

Recombinant full-length human DHTR protein
Antibody target cellular localization

Cytoplasmic, Nucleus
Verified antibody applications

IHC (FFPE) (verified)
Species reactivity

Human
Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Does not react with mouse; others not known, Optimal dilution for a specific application should be determined by user
Positive control

LNCap cells or Prostate carcinoma
Shipping condition

Room temperature
Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status

For research use only (RUO)
Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas

Cancer, Endocrinology, Signal transduction
Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Tumor expression

Prostate cancer

Technology

CF® Dyes
Considerations for Immunofluorescence Staining
Primary & Secondary Antibody Conjugates
Protocols
SDS
Supporting Documents
Download Datasheet

Product Description

This antibody recognizes a protein of 110 kDa, which is identified as androgen receptor (AR). It reacts with full length, and the newly described A form of the receptor. It does not cross react with estrogen, progesterone, or glucocorticoid receptors. AR functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. The expression of AR is reportedly inversely correlated with histologic grade; ie, well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed as a marker of hormone-responsiveness and thus it may be useful in identifying patients likely to benefit from anti-androgen therapy. Anti-androgen receptor has been useful clinically in differentiating morpheaform basal cell carcinoma (mBCC) from desmoplastic trichoepithelioma (DTE) in the skin.This MAb is superb for staining of formalin/paraffin tissues.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@biotrend.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0882, Anti-Androgen Receptor (DHTR/882) 

Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405 CF®405S Features
BNC88 CF®488A 490/515 488 GFP, FITC CF®488A Features
BNC68 CF®568 562/583 532, 561 RFP, TRITC CF®568 Features
BNC94 CF®594 593/614 561 Texas Red® CF®594 Features
BNC40 CF®640R 642/662 633-640 Cy®5 CF®640R Features
BNC47 CF®647 650/665 633-640 Cy®5 CF®647 Features
BNC74 CF®740 742/767 633-685 775/50 CF®740 Features
BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free		 N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

Note: Listed references are for this antibody clone sold by Biotium and other suppliers.