AM4-66

Katalog-Nummer 70050

Size : 1MG

Marke : Biotium

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Telefonnummer : +1 850 650 7790

Probe cellular localization

Membrane/vesicular

For live or fixed cells

For live/intact cells

Assay type/options

Real-time imaging

Fixation options

Fix after staining (formaldehyde), Permeabilize after staining

Colors

Far-red

Excitation/Emission

510/750 nm (see product description)

Product Description

AM4-66 is a fixable red fluorescent nerve terminal probe. The dye is spectrally identical to SynaptoRed™ C2 (also called FM®4-64), but it has an additional amine group at the hydrophilic end of the molecule, making the dye fixable with formaldehyde or gluteraldehyde. The long emission wavelength makes the dye suitable to be used with GFP in two-color imaging.

  • Red fluorescent nerve terminal probe that is fixable with formaldehyde or gluteraldehyde
  • λExEm (membranes) = ~510/750 nm*
  • Dark purple solid soluble in water
  • MW: 872.85

* Excitation/emission settings of 515/640 nm have been used for detection of yeast vacuole staining with SynaptoRed™ C2 (FM®4-64), and should be applicable to AM4-66.

Nerve Terminal Dyes and Kits

Product Catalog No. Features
SynaptoGreen™ Dyes
(Ex/Em ~480/660 nm in membranes)
70020... 70053

View full list of SynaptoGreen™ Dyes

SynaptoRed Dyes™
(Ex/Em ~510/750 nm in membranes)
70021... 70050

View full list of SynaptoRed™ Dyes

Background Reducers

ADVASEP-7

70029

Sulfonated cyclodextrin that aids in removal of free dye during washes

SCAS

70037

Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7

Sulforhodamine 101

80101

Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes
Nerve Terminal Staining Kits

Nerve Terminal Staining Kit I

70030

Includes SynaptoGreen™ C4 and ADVASEP-7

Nerve Terminal Staining Kit II (A)

70031

Includes AM1-43 and ADVASEP-7

Nerve Terminal Staining Kit II (B)

70031-1

Includes AM1-43 and SCAS

Nerve Terminal Staining Kit III

70032

Includes SynaptoGreen™ C4 and Sulforhodamine 101

Nerve Terminal Staining Kit V

70034

Includes SynaptoRed™ C2 and ADVASEP-7
m is the number of carbons in the lipophilic tail and n is the number of double bonds linking the two aromatic rings in the dye.
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.
 

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