Western blot: 1/1,000-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/4,000-1/20,000. Immunohistochemistry on Paraffin (FFPE) or Frozen sections: 1/500-1/2,500.
Reactivity
Yeast
Antibody Host
Rabbit
Clonality
Polyclonal
Immunogen
Alcohol dehydrogenase from yeast
Specificity
This antibody detects yeast alcohol dehydrogenase. Cross reactivity against alcohol dehydrogenase from other sources may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified yeast alcohol dehydrogenase.
Buffer
0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig faction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method
Restore with 0.1 ml of deionized water (or equivalent).
Concentration
lot specific
Purification
Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation
HRP
Storage
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Alcohol Dehydrogenases (ADH) are a group of dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of nicotinamide adenine dinucleotide (NAD+ to NADH). In humans and many other animals, they serve to break down alcohols that otherwise are toxic, and they also participate in generation of useful aldehyde, ketone, or alcohol groups during biosynthesis of various metabolites.
Synonyms
Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, YOL086C, O0947
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