Acridine Orange (zinc chloride salt) [10127-02-3]
Katalog-Nummer HY-D0942-100mg
Size : 100mg
Marke : MedChemExpress
Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm).
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Acridine Orange zinc chloride salt Chemische Struktur
CAS. Nr. : 10127-02-3
This product is a controlled substance and not for sale in your territory.
Based on 21 publication(s) in Google Scholar
Other Forms of Acridine Orange zinc chloride salt:
- Acridine Orange hydrochloride In-stock
- Acridine Orange base Angebot einholen
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Acridine Orange zinc chloride salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Jul 30;11(7):607. [Abstract]
- Depending on the acidity, Acridine Orange hydrochloride (AO) causes autophagic lysosomes to appear as orange/red fluorescent vesicles, while it caused nuclei to appear green. AO staining demonstrates that LA dose-dependently increased the numbers of intracellular acidic compartments.
Alle Parasite Isoform-spezifische Produkte anzeigen:
Beschreibung |
Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3]. |
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In Vitro |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[4]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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In Vivo |
Acridine Orange (0.1 mg/kg, i.v., dogs) zinc chloride salt shows no clinical signs of toxicity and no abnormalities were seen in the blood within 30 days[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molekulargewicht |
438.10 |
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Formel |
C17H20Cl3N3Zn |
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CAS. Nr. |
10127-02-3 |
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Appearance |
Solid |
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Color |
Brown to reddish brown |
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SMILES |
Cl[Zn]Cl.CN(C)C1=CC=C2C(N=C3C=C(N(C)C)C=CC3=C2)=C1.Cl |
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Versand | Room temperature in continental US; may vary elsewhere. |
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Speicherung |
4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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Lösungsmittel & Löslichkeit |
In Vitro:
DMSO : 100 mg/mL (228.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Reinheit & Dokumentation |
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Verweise |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 2.2826 mL | 11.4129 mL | 22.8258 mL | 57.0646 mL |
5 mM | 0.4565 mL | 2.2826 mL | 4.5652 mL | 11.4129 mL | |
10 mM | 0.2283 mL | 1.1413 mL | 2.2826 mL | 5.7065 mL | |
15 mM | 0.1522 mL | 0.7609 mL | 1.5217 mL | 3.8043 mL | |
20 mM | 0.1141 mL | 0.5706 mL | 1.1413 mL | 2.8532 mL | |
25 mM | 0.0913 mL | 0.4565 mL | 0.9130 mL | 2.2826 mL | |
30 mM | 0.0761 mL | 0.3804 mL | 0.7609 mL | 1.9022 mL | |
40 mM | 0.0571 mL | 0.2853 mL | 0.5706 mL | 1.4266 mL | |
50 mM | 0.0457 mL | 0.2283 mL | 0.4565 mL | 1.1413 mL | |
60 mM | 0.0380 mL | 0.1902 mL | 0.3804 mL | 0.9511 mL | |
80 mM | 0.0285 mL | 0.1427 mL | 0.2853 mL | 0.7133 mL | |
100 mM | 0.0228 mL | 0.1141 mL | 0.2283 mL | 0.5706 mL |
Acridine Orange zinc chloride salt Related Classifications
- Biochemische Testreagenzien
- Others
- Others
- Anti-infection Others Cell Cycle/DNA Damage
- Parasite Fluorescent Dye DNA Stain