Acetyl CoA synthetase (ACSS2) rabbit polyclonal antibody, Biotin
Specifications
Product Data
Application
ELISA, ID, IF, IP, R, WB
Recommended Dilution
This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/1,000-1/10,000).
Reactivity
Bakers Yeast
Antibody Host
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
Purified S-Acetyl Coenzyme A Synthetase from baker's Yeast
Specificity
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Buffer
PBS, pH 7.2 without preservatives and foreign proteins. Label: Biotin State: Lyophilized IgG fraction. Label: Conjugation Procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life Molar radio: ~5.5
Reconstitution Method
Restore by adding 1.0 ml of sterile distilled water.
Concentration
lot specific
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Conjugation
Biotin
Storage
Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Acetyl Coenzyme A Synthetase is a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. It acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
