Images
Immunocytochemistry
Application

Immunocytochemistry

Immunocytochemical staining of HEK293 with 4-Hydroxynonenal monoclonal antibody, clone 12F7 (Cat # MAB19282). (A, B) DAPI (blue) nuclear stain, (C, D) Phalloidin Alex Fluor 633 F-Actin stain, (E, F) 4-Hydroxynonenal Antibody and (G, H) Composite. (A, C, E, G) Untreated and (B, D, F, H) Cells cultured overnight with 50 uM H2O2.

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against synthetic 4-Hydroxynonenal (4-HNE).

    Immunogen

    Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).

    Host

    Mouse

    Reactivity

    Human

    Form

    Liquid

    Conjugation

    ATTO 488

    Purification

    Protein G Purified

    Isotype

    IgG1

    Recommend Usage

    ELISA (1:1000)
    Immunocytochemistry (1:50)
    Immunofluorescence (1:50)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).

    Storage Instruction

    Store at -20°C.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Immunocytochemistry

    Immunocytochemical staining of HEK293 with 4-Hydroxynonenal monoclonal antibody, clone 12F7 (Cat # MAB19282). (A, B) DAPI (blue) nuclear stain, (C, D) Phalloidin Alex Fluor 633 F-Actin stain, (E, F) 4-Hydroxynonenal Antibody and (G, H) Composite. (A, C, E, G) Untreated and (B, D, F, H) Cells cultured overnight with 50 uM H2O2.

    Immunofluorescence

    Enzyme-linked Immunoabsorbent Assay