(S)-mchm5U [89665-83-8]

(S)-mchm5U is a hydroxylated form of mcm5U, may be present in tRNA from the silkworm Bombyx mori. (S)-mchm5U may be present also in mammalian tRNA, and that the ALKBH8 oxygenase could be responsible for its formation. (S)-mchm5U can be used for RNA modification synthesis^[1].

332.26

C₁₂H₁₆N₂O₉

89665-83-8

Solid

White to off-white

O=C(N(C=C1[C@@H](C(OC)=O)O)[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)NC1=O

Room temperature in continental US; may vary elsewhere.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. van den Born E, et al. ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA. Nat Commun. 2011 Feb 1;2:172.  [Content Brief]